William Yards. Geisler, Robert J. Suchland, William L. H. Whittington, Walter E. Stamm, Quantitative Culture out of Chlamydia trachomatis: Matchmaking out of Addition-Developing Tools Manufactured in Culture to Scientific Signs and you will Severe Pain during the Urogenital State, This new Record regarding Infectious Disease, Volume 184, Procedure ten, , Profiles 1350–1354,
Abstract
The connection off Chlamydia trachomatis introduction-forming units in quantitative culture so you’re able to medical manifestations and you can inflammation inside the urogenital situation try assessed within the 1179 patients gonna an intimately carried infection clinic. 450 ifu), amount and you may character of cervical release, ?30 polymorphonuclear tissue (PMNL) for every single higher-strength occupation (hpf) to the Gram spot (2050 against. 320 ifu), and you can diagnoses out of mucopurulent cervicitis (MPC; 2550 versus. three hundred ifu) and you may pelvic inflammatory condition (PID; 3000 versus. 578 ifu). During the men, better inclusion-developing product matters had been in the urethral discharge (85 vs. 49 ifu), number and you will reputation from launch, and you can ?10 PMNL/hpf https://www.datingranking.net/nl/xdating-overzicht/ (95 compared to. fifty ifu). These types of connectivity proceeded towards multivariate research. Ergo, chlamydial replication is for the MPC and you will PID in women, urethritis within the guys, and you will pain in. Because infection with high inclusion matters will be the very transmissible, character and you may treatment of clients with our chlamydia-relevant syndromes leads to manage software
Understanding the matchmaking regarding Chlamydia trachomatis urogenital situation in order to systematic symptoms and you can serious tenderness is important in most readily useful knowing the epidemiology and you can pathogenesis of those problems. Clinical symptoms due to C. trachomatis likely represent the brand new combined results of lead chlamydial replication and servers inflammatory answers [ 1]. The partnership anywhere between productive chlamydial duplication and you will clinical symptoms are analyzed utilising the quantity of inclusion-forming systems manufactured in mobile cells community because a quantitative level of level of viable infectious primary regulators inside vaginal secretions. What number of introduction-developing gadgets when you look at the society can certainly be thought to be good surrogate getting infectivity otherwise transmissibility [ 2]. From the use of a large database one to acceptance to possess improvement out-of possible confounding things, i used a combination-sectional study to evaluate the relationship between chlamydial addition-building gadgets within the quantitative societies that have scientific symptoms and you can level of serious pain in urogenital chlamydial problems
Tips
Diligent populace and you may studies collectionThe analysis populace consisted of 671 female and you will 933 heterosexual boys who had a culture-documented urogenital chlamydial infection during the Societal Wellness, Seattle and you will Queen County Sexually Transmitted Problems Clinic away from Could possibly get 1995 courtesy December 1999. Research studies was indeed about clinic’s computerized databases, which includes intercourse-certain standardized scientific list versions. Individuals having coexisting gonococcal issues or attacks because of nonfusing C. trachomatis phenotypes was in fact excluded as an easy way regarding dealing with potential confounders one to ydial introduction-creating tool matters so you can systematic manifestations [ step three, 4]. Customers having recite problems with the exact same serovar contained in this thirty days or blended serovar problems have been excluded, as their inclusion perform break liberty throughout the multivariate analysis
Source and characterization ofC. trachomatis isolatesThe methods used for specimen collection, isolation of C. trachomatis identification and quantification of inclusions, and serotyping have been described in detail elsewhere [ 2, 5]. In brief, specimens were collected, were stored in chlamydia transport medium at 4°C, and were transported within 24 h to the laboratory. Each specimen was inoculated onto McCoy cells and overlaid with growth medium. Chlamydial inclusions were detected with a genus-specific antichlamydial lipopolysaccharide monoclonal antibody conjugated with fluorescein. The average inclusion count per 100-?L well was determined by counting 3 fields and multiplying the average count by a predetermined conversion factor based on magnification with a Zeiss epifluorescence microscope. Inclusions were counted individually in cultures with <100 inclusions. All technicians who determined inclusion-forming unit counts used the same standardized method [ 5]. For serotyping, chlamydial strains were cultured in 96-well microtiter plates and were serotyped by using subspecies- and serovar-specific monoclonal antibodies. Serovars were additionally grouped as B complex (serovars B, Ba, D, Da, D ? , E, L1, L2, and L2a), intermediate group FG (serovars F and G), and C complex (serovars A, C, H, I, Ia, I ? , J, K, and L3) [ 6–8]. The nonfusing chlamydial phenotype was detected visually by observing inclusion morphology during serotyping [ 5]
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